72ANTI MICROBIAL AND IN-VITRO ACTIVITIES OF PLANTSemecarpus anacardium (SA) Linn (Family: Anacardiacea)To evaluate the biological and antitumor activities of Semecarpus anacardium Linn. Four solvent extracts (ethyl acetate, acetone, chloroform, water) of Semecarpus anacardium Plant parts were investigated against Staphylococcus aureus, Salmonella typhii, Escherichia coli, Bacillus subtilis, Aeromonas hydrophila and Vibrio cholerae by using agar well diffusion method. Candida albicans, Cryptococcus neoformans, Trichophyton mentagrophytes and Epidermophyton species were used to test anti-yeast and anti-fungal activity. The cytotoxic effects of the extracts on Vero and HEp2 cells were assayed using MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. Results: Among the four extracts tested, ethanol extract had effective antibacterial potential, followed by ethyl acetate extract. The ethanol extract showed greater activity against gram positive organism than against gram negative. All the extracts were active against Epidermophyton species and Candida albicans. The 1:8 dilution of the acetone extract was non-toxic to normal cells and also had both anticancer and antiproliferative activity against cancerous cells. Conclusion: The study confirms the biological and antitumor activity of solvent extracts of Semecarpus anacardium Plant part, which would serve as a potent drug in near future.yuvas2010@gmail.comResearch432014Semecarpus anacardium,Antitumor,HEp2 cells, MTT assayK. Senthil kumar,R. VasukiDepartment of Bio-Medical Engineering, Bharath Institute of Science & Technology, Bharath University, Chennai - 600073, Tamilnadu, India.,Department of Bio-Medical Engineering, Bharath Institute of Science & Technology, Bharath University, Chennai - 600073, Tamilnadu, India.