59TOXICITY STUDY OF DIOSPYROS MONTANA IN JUVENILE ZEBRA FISHPlants can be used to catch the fish. A study was conducted to evaluate the toxicity of fresh leaves of Diospyros montanain fish and to evaluate some phytochemical constituents. Fresh leaves were weighed, finely ground and constituted to 50 g/L in water. LC50 study was conducted using Zebra-fish based on a preliminary range finding test. Serial concentrations were employed for the study i.e., 0.5, 1.0, 1.5, 2.0 and 2.25 g/L, each concentration was tested in a container with 10 fishes in 4 liters of water to identify the LC50 and it was found to be 1.37 g/L. Steroids and alkaloids were present in petroleum ether and methanol extracts respectively. Fresh leaves were negative for the presence of nitrate and cyanide on diphenylamine test and picrate paper test respectively. Diospyros Montana was toxic to fish, this property of the plant can be used to kill predatory fish in shrimp culture and pisciculture instead of ecotoxic chemicals.drdrarunraj26@gmail.comResearch422014Diospyros montana, Zebra fish,Nitrate,Cyanide,PhytochemistryShridhar NB, Santhosh Kumar CN,Ambika Das,Arun Raj GRAssociate Professor,Department of Veterinary Pharmacology and Toxicology, Veterinary College, KVAFSU, Hebbal, Bangalore- 560 024, Karnataka, India.,PhD Scholar, Department of Veterinary Pharmacology and Toxicology, Veterinary College, KVAFSU, Hebbal, Bangalore- 560 024, Karnataka, India.,Post Graduate Scholar, Department of Post graduate Studies in Kaumarabhritya, Shri Dharmasthala Manjunatheshwara College of Ayurveda and Hospital, Hassan, Karnataka, India.,Post Graduate Scholar, Department of Post graduate Studies in Kaumarabhritya, Shri Dharmasthala Manjunatheshwara College of Ayurveda and Hospital, Hassan, Karnataka, India.