255INVESTIGATION ON ANALGESIC ACTIVITY AND WOUND HEALING ACTIVITY OF SPHAGNETICOLA CALENDULACEA – A PROSPECTIVE STUDYWound healing is a complex and dynamic biological process involving inflammation, tissue formation, and remodeling, which is essential for restoring the integrity of damaged skin. This study aims to evaluate the wound healing and analgesic potential of Sphagneticola calendulacea methanol extract (MESC) through phytochemical screening, acute toxicity assessment, and in vivo wound healing models. Phytochemical analysis confirmed the presence of flavonoids,alkaloids,tannins,terpenoids and glycosides,which are known for their antioxidant,anti-inflammatory,antimicrobial and tissue regenerative properties.the acute oral toxicity study,conducted as per OECD guidelines,indicated that MESCis non-toxic at therapeutic doses,pharmacological investigations . To assess its wound healing efficacy, excision and incision wound models were used in Wistar rats, where the topical application of MESC resulted in significantly enhanced wound contraction rates, reduced epithelialization period and improved histopathological tissue regeneration. when compared to the standard povidone -iodine treatment, MESC exhibited comparable or superior healing effects, demonstrating its potential as a natural alternative. Additionally, the analgesic properties of MESC were evaluated using tail immersion and hot plate methods, which are widely accepted for pain assessment in preclinical studies.the extract showed significant analgesic activity, indicating its potential in pain management wound healing, which could reduce dependency on synthetic NSAIDs. Overall,the findings of this study highlight Sphagneticola calendulacea as promising candidate for wound management, possessing both healing and analgesic benefits.jothies82@gmail.comResearch1512025Methanol extract of Sphagneticola calendulacea (MESC), Indian System of Medicine (ISM)Rahul T, Ragini V, Ravi Kiran D, Jeevana K, M Uma, Jothieswari DSri Venkateswara College of Pharmacy (Autonomous), RVS Nagar, Tirupati Road, Chittoor-517127. Andhra Pradesh, India. Sri Venkateswara Pharmacy College, RVS Nagar, Tirupati Road, Chittoor-517127. Andhra Pradesh, India