234NUTRIENT ASSOCIATIONS WITH PARAOXONASE 1 ACTIVITY: INSIGHTS FROM A CARDIOVASCULAR DISEASE COHORT STUDYThis study aimed to investigate the association between paraoxonase 1 (PON1) activity and various nutrients, beyond vitamin C and E, in the context of cardiovascular disease risk. Utilizing data from the Carotid Lesion Epidemiology and Risk cohort, 2804 subjects underwent standardized food frequency surveys to assess nutrient intake. Stepwise regression analysis revealed independent associations between PON1 arylesterase activity and vitamin C, iron, folic acid, dietary cholesterol, and alcohol intake. Notably, dietary cholesterol explained 6.5% of the variance in PON1 activity. These findings underscore the significance of cholesterol, iron, and folic acid in modulating PON1 activity, complementing previous research. Understanding the factors influencing PON1 activity is crucial for elucidating its role in human disease, particularly in relation to high-density lipoprotein (HDL) functionality and cardiovascular healthSatyanarayana@gmail.comResearch1222022 Paraoxonase 1 (PON1), Cardiovascular disease, Nutrient intake, Dietary cholesterol, HDL functionalityDr Satyanarayana YandaAssistant professor, Department of General Medicine, Viswabarathi Medical College & General Hospital, RT Nagar, Penchikalapadu, Kurnool, India.