165EFFECT OF Artocarpus heterophyllus PHENOLIC SEED EXTRACT IN ANIMAL MODELS OF ANXIETY: A PRECLINICAL STUDYMany indigenous plants with anti oxidant property have shown to be beneficial in many behavioral disorders like anxiety. Objective: To evaluate anxiolytic activity of Artocarpus Heterophyllus phenolic seed extract (AHPSE) and to screen for it’s the possible potentiating anxiolytic activity with Diazepam in Swiss albino mice. Methodology: A total of 84 healthy male Swiss albino mice weighing 25-35 g. were used and they were divided into fourteen groups of six mice in each. First seven groups (1st -7th) were evaluated by Light and Dark Arena (LDA) and remaining by Elevated Plus Maze (EPM) for anxiolytic action. 1st group (control) received normal saline 10mg/kg, 2nd group (standard) Diazepam 1mg/kg, 3rd , 4th, 5th , 6th and 7th groups (test) respectively received AHPSE in different doses 100mg, 200mg, 400mg, 800 mg and 800mg/kg + 1mg/Kg Diazepam per orally. They were evaluated for anxiolytic activity using LDA after 60 minutes of drug administration. Similarly, remaining seven groups received the same drugs and evaluated using EPM after 60 minutes of drug administration. The duration of time spent and numbers of entries in light compartment/open arm and dark compartment/closed arm were noted for five minutes for each mouse. Results: One way ANOVA followed by Tukey-Kramer multiple comparison test clearly showed that AHPSE in all the doses (100-800mg/kg) has shown increase in the time spent in light compartment/open arm and decrease in the time spent in the dark compartment/closed arm when compared to control and standard groups (p<0.001) in both LDA and EPM models. The study also proved potentiating activity of AHPSE in both the models when combined with Diazepam with p value <0.001. Conclusion: The current study has demonstrated an anxiolytic and potentiating effect of AHPSE in animal models of anxietynpbhat17@gmail.comResearch1012020Artocarpus Heterophyllus phenolic seed extract,Elevated plus maze, light and dark arenaPrabhakar Adake,Mahalaxmi S Petimani,Nagapati Prabhakar BhatAssociate Professor of Pharmacology, Yenepoya Medical College, Yenepoya (Deemed to be University), Deralakatte, Mangalore-575018, Karnataka, India,Assistant Professor of Biochemistry, Yenepoya Medical College, Yenepoya (Deemed to be University), Deralakatte, Mangalore-575018, Karnataka, India,Assistant Professor of Pharmacology, Yenepoya Medical College, Yenepoya (Deemed to be University), Deralakatte, Mangalore-575018, Karnataka, India